Application Notes

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In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.

In Vitro 3D Lung Cancer Model Presents a More Relevant Expression of Junctional Proteins than 2D Cultures (English)

In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.
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We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.

Miniaturization and Automation of a full-length Single-Cell RNA-seq Workflow (English)

We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.
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A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.

Advanced In Vitro 3D Models to Investigate iPSC Pluripotency and Capillary Network Formation of HUVECs (English)

A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.
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Our study demonstrates that the c.bird™ improves mammalian cell growth in 96-well culture environments and closely imitates the shaker-flask culture in terms of cell growth profiles and protein yields.

Providing Optimal Suspension Culture Conditions in 96-well Plates and Superior Comparability with Large-scale Shaker-flask Culture Environments

Our study demonstrates that the c.bird™ improves mammalian cell growth in 96-well culture environments and closely imitates the shaker-flask culture in terms of cell growth profiles and protein yields.
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In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.

3D Bioprinting Skin Tissue Models Using Primary Cells

In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.
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In this application note, we explore the use of live-cell imaging to determine proliferation of label-free cells as well as fluorescently labeled cells.

Determination of Cell Proliferation Using the CELLCYTE X Live-cell Imaging System

In this application note, we explore the use of live-cell imaging to determine proliferation of label-free cells as well as fluorescently labeled cells.
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In this technical note, we explore cell proliferation using the CELLCYTE XTM live-cell imaging system to conduct time-course experiments.

Cell Proliferation Using Live-cell Imaging: A Technical Note

In this technical note, we explore cell proliferation using the CELLCYTE XTM live-cell imaging system to conduct time-course experiments.
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Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X.

Evaluating Liver Toxicity in Bioprinted Mini Livers

Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X.
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Assessing the effectiveness of the I-DOT, a low-volume noncontact liquid dispensing device, to form spheroids using the liquid overlay technique.

Tumor Spheroid Formation Using the I-DOT

Assessing the effectiveness of the I-DOT, a low-volume noncontact liquid dispensing device, to form spheroids using the liquid overlay technique.
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Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models.

Comparing Drug Response in 2D Cultures and 3D Bioprinted Tumoroids

Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models.
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3D bioprinting a syngeneic tumor model of murine lung cancer on the BIO X to evaluate an immune checkpoint inhibitor (PD-1) in a 3D T cell cytotoxicity assay.

3D Bioprinted Tumor Model for Immuno-oncology Applications

3D bioprinting a syngeneic tumor model of murine lung cancer on the BIO X to evaluate an immune checkpoint inhibitor (PD-1) in a 3D T cell cytotoxicity assay.
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