Application Notes

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The ability to dispense liquids into the plates afterward enables the fully automated and reproducible washing of entire microwell plates in a high-throughput manner. Here, we determined washing efficiencies achieved by the C.WASH for different rotational speeds in 96-well and 384-well microplate formats. |

Highly Efficient Washing of Microwell Plates Using Centrifugal Forces

| The ability to dispense liquids into the plates afterward enables the fully automated and reproducible washing of entire microwell plates in a high-throughput manner. Here, we determined washing efficiencies achieved by the C.WASH for different rotational speeds in 96-well and 384-well microplate formats.
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In this acellular proof-of-concept study, a five-layer skin model, which comprises extruded dermal layers, coaxial printed vascular channels, precise hair follicle dispensing, and noncontact epidermal coating, was printed using the BIO X6. |

Optimized Bioprinting of Advanced Tissue Models with the BIO X6

| In this acellular proof-of-concept study, a five-layer skin model, which comprises extruded dermal layers, coaxial printed vascular channels, precise hair follicle dispensing, and noncontact epidermal coating, was printed using the BIO X6.
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The fact that the majority of microbial organisms remain unexplored holds enormous potential to discover new metabolic pathways that can be applied in biotechnological processes or used to develop new therapeutics. We present a workflow for exploring such microbial dark matter, based on single-cell isolation and genetic characterization. | |

Sequencing Single-cell Genomes of Uncultivated Bacteria in the Human Oral Microbiome

| | The fact that the majority of microbial organisms remain unexplored holds enormous potential to discover new metabolic pathways that can be applied in biotechnological processes or used to develop new therapeutics. We present a workflow for exploring such microbial dark matter, based on single-cell isolation and genetic characterization.
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Clonal cell line development is a crucial step for biopharmaceutical generation (e.g., the production of monoclonal antibodies). To ensure a consistent product, the cell lines used in production must originate from a single cell. |

Combined Single-cell Dispensing and 3D Full Well Imaging for Cell Lines with >99.99% Probability of Monoclonality (English)

| Clonal cell line development is a crucial step for biopharmaceutical generation (e.g., the production of monoclonal antibodies). To ensure a consistent product, the cell lines used in production must originate from a single cell.
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The long-distance transportation of mammalian cells usually requires cryo-preservation in dry ice or liquid nitrogen. Both of these methods are hazardous in nature and associated with high shipping costs. Here, we present the cost-effective shipping of 3D bioprinted constructs at ambient temperature using a novel encapsulation technology WellReady™ from Atelerix. |

Room-temperature Transport of 3D Bioprinted Constructs Using WellReady™ In-plate Preservation

| The long-distance transportation of mammalian cells usually requires cryo-preservation in dry ice or liquid nitrogen. Both of these methods are hazardous in nature and associated with high shipping costs. Here, we present the cost-effective shipping of 3D bioprinted constructs at ambient temperature using a novel encapsulation technology WellReady™ from Atelerix.
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We demonstrate an efficient, high-throughput workflow that incorporates the f.sight™ for both selection and cloning of CRISPR/Cas9-edited mesenchymal stem cells (MSCs). Here, MSCs underwent gene editing to knockout the RANKL protein to further enhance the therapeutic capacity of MSCs in regenerative medicine. |

Streamlined Workflow for the Generation of CRISPR-edited Mesenchymal Stem Cell Clones for Regenerative Medicine Applications

| We demonstrate an efficient, high-throughput workflow that incorporates the f.sight™ for both selection and cloning of CRISPR/Cas9-edited mesenchymal stem cells (MSCs). Here, MSCs underwent gene editing to knockout the RANKL protein to further enhance the therapeutic capacity of MSCs in regenerative medicine.
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This study demonstrates that the c.bird improves mammalian cell line culture conditions over static culture in the early stages of CLD.

C.BIRD – A microbioreactor that enables suspension culture in 96-well plates for improved cell growth and recombinant protein yield

This study demonstrates that the c.bird improves mammalian cell line culture conditions over static culture in the early stages of CLD.
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Here we introduce a simple workflow for rapid generation of axenic cultures of gut bacteria and generated 1,181 clonal cultures of Escherichia coli and Bacteroides vulgatus and 1,666 clonal cultures of mouse gut bacteria. In the ultimate cultures, MALDI-TOF-MS identified up to 500 isolates, revealing the presence of potentially new species. |

B.SIGHT – High-throughput Cultivation Workflow for Isolation of Anaerobic Gut Bacteria

| Here we introduce a simple workflow for rapid generation of axenic cultures of gut bacteria and generated 1,181 clonal cultures of Escherichia coli and Bacteroides vulgatus and 1,666 clonal cultures of mouse gut bacteria. In the ultimate cultures, MALDI-TOF-MS identified up to 500 isolates, revealing the presence of potentially new species.
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Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations. |

Printing Alginate Beads: A Technical Note

| Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations.
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In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins. | |

In Vitro 3D Lung Cancer Model Presents a More Relevant Expression of Junctional Proteins than 2D Cultures (English)

| | In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.
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We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.

Miniaturization and Automation of a full-length Single-Cell RNA-seq Workflow (English)

We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.
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A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days. |

Advanced In Vitro 3D Models to Investigate iPSC Pluripotency and Capillary Network Formation of HUVECs (English)

| A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.
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