A 3D Bioprinted Model to Study Osteogenic Differentiation of Primary Mesenchymal Stem Cells

In the bioprinting field, the use of primary human mesenchymal stem cells (hMSCs) is considered a gold standard for the initial evaluation of bioink compatibility and the bioprinting process. This is done by determining the viability and functionality of the cells. hMSCs are also used in the bone tissue regeneration, because of the cells’ potential to differentiate into different cell lineages.

The aim of this application note is to develop a 3D bioprinted model with which we can study osteogenic differentiation of hMSCs. The goal is to have this 3D model recapitulate the in vivo microenvironment.

The cells are mixed with GelMA Fibrin and bioprinted in multi-well plates using the BIO X, where the bioink is mixed with UV light and thrombin.