Application notes

Written by scientists for scientists, these papers cover a wide range of applications and highlight novel ways to optimize one’s research with our devices, technologies and consumables.

A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days. |

Advanced In Vitro 3D Models to Investigate iPSC Pluripotency and Capillary Network Formation of HUVECs

| A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.
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Our study demonstrates that the c.bird™ improves mammalian cell growth in 96-well culture environments and closely imitates the shaker-flask culture in terms of cell growth profiles and protein yields. |

Providing Optimal Suspension Culture Conditions in 96-well Plates and Superior Comparability with Large-scale Shaker-flask Culture Environments

| Our study demonstrates that the c.bird™ improves mammalian cell growth in 96-well culture environments and closely imitates the shaker-flask culture in terms of cell growth profiles and protein yields.
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In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis. |

3D Bioprinting Skin Tissue Models Using Primary Cells

| In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.
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In this application note, we explore the use of live-cell imaging to determine proliferation of label-free cells as well as fluorescently labeled cells. |

Determination of Cell Proliferation Using the CELLCYTE X Live-cell Imaging System

| In this application note, we explore the use of live-cell imaging to determine proliferation of label-free cells as well as fluorescently labeled cells.
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In this technical note, we explore cell proliferation using the CELLCYTE XTM live-cell imaging system to conduct time-course experiments. |

Cell Proliferation Using Live-cell Imaging: A Technical Note

| In this technical note, we explore cell proliferation using the CELLCYTE XTM live-cell imaging system to conduct time-course experiments.
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We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT. | |

Miniaturization and Automation of a full-length Single-Cell RNA-seq Workflow

| | We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.
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Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X. |

Evaluating Liver Toxicity in Bioprinted Mini Livers

| Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X.
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Assessing the effectiveness of the I-DOT, a low-volume noncontact liquid dispensing device, to form spheroids using the liquid overlay technique. |

Tumor Spheroid Formation Using the I-DOT

| Assessing the effectiveness of the I-DOT, a low-volume noncontact liquid dispensing device, to form spheroids using the liquid overlay technique.
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Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models. |

Comparing Drug Response in 2D Cultures and 3D Bioprinted Tumoroids

| Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models.
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3D bioprinting a syngeneic tumor model of murine lung cancer on the BIO X to evaluate an immune checkpoint inhibitor (PD-1) in a 3D T cell cytotoxicity assay. |

3D Bioprinted Tumor Model for Immuno-oncology Applications

| 3D bioprinting a syngeneic tumor model of murine lung cancer on the BIO X to evaluate an immune checkpoint inhibitor (PD-1) in a 3D T cell cytotoxicity assay.
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