Application notes

Written by scientists for scientists, these papers cover a wide range of applications and highlight novel ways to optimize one’s research with our devices, technologies and consumables.

The long-distance transportation of mammalian cells usually requires cryo-preservation in dry ice or liquid nitrogen. Both of these methods are hazardous in nature and associated with high shipping costs. Here, we present the cost-effective shipping of 3D bioprinted constructs at ambient temperature using a novel encapsulation technology WellReady™ from Atelerix.

Room-temperature Transport of 3D Bioprinted Constructs Using WellReady™ In-plate Preservation

The long-distance transportation of mammalian cells usually requires cryo-preservation in dry ice or liquid nitrogen. Both of these methods are hazardous in nature and associated with high shipping costs. Here, we present the cost-effective shipping of 3D bioprinted constructs at ambient temperature using a novel encapsulation technology WellReady™ from Atelerix.
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We demonstrate an efficient, high-throughput workflow that incorporates the f.sight™ for both selection and cloning of CRISPR/Cas9-edited mesenchymal stem cells (MSCs). Here, MSCs underwent gene editing to knockout the RANKL protein to further enhance the therapeutic capacity of MSCs in regenerative medicine.

Streamlined Workflow for the Generation of CRISPR-edited Mesenchymal Stem Cell Clones for Regenerative Medicine Applications

We demonstrate an efficient, high-throughput workflow that incorporates the f.sight™ for both selection and cloning of CRISPR/Cas9-edited mesenchymal stem cells (MSCs). Here, MSCs underwent gene editing to knockout the RANKL protein to further enhance the therapeutic capacity of MSCs in regenerative medicine.
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This study demonstrates that the c.bird improves mammalian cell line culture conditions over static culture in the early stages of CLD.

c.bird – A microbioreactor that enables suspension culture in 96-well plates for improved cell growth and recombinant protein yield

This study demonstrates that the c.bird improves mammalian cell line culture conditions over static culture in the early stages of CLD.
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Here we introduce a simple workflow for rapid generation of axenic cultures of gut bacteria and generated 1,181 clonal cultures of Escherichia coli and Bacteroides vulgatus and 1,666 clonal cultures of mouse gut bacteria. In the ultimate cultures, MALDI-TOF-MS identified up to 500 isolates, revealing the presence of potentially new species.

b.sight – High-throughput Cultivation Workflow for Isolation of Anaerobic Gut Bacteria

Here we introduce a simple workflow for rapid generation of axenic cultures of gut bacteria and generated 1,181 clonal cultures of Escherichia coli and Bacteroides vulgatus and 1,666 clonal cultures of mouse gut bacteria. In the ultimate cultures, MALDI-TOF-MS identified up to 500 isolates, revealing the presence of potentially new species.
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Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations.

Printing Alginate Beads: A Technical Note

Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations.
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In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.

In Vitro 3D Lung Cancer Model Presents a More Relevant Expression of Junctional Proteins than 2D Cultures

In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.
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A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.

Advanced In Vitro 3D Models to Investigate iPSC Pluripotency and Capillary Network Formation of HUVECs

A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.
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Our study demonstrates that the c.bird™ improves mammalian cell growth in 96-well culture environments and closely imitates the shaker-flask culture in terms of cell growth profiles and protein yields.

Providing Optimal Suspension Culture Conditions in 96-well Plates and Superior Comparability with Large-scale Shaker-flask Culture Environments

Our study demonstrates that the c.bird™ improves mammalian cell growth in 96-well culture environments and closely imitates the shaker-flask culture in terms of cell growth profiles and protein yields.
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In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.

3D Bioprinting Skin Tissue Models Using Primary Cells

In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.
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In this application note, we explore the use of live-cell imaging to determine proliferation of label-free cells as well as fluorescently labeled cells.

Determination of Cell Proliferation Using the CELLCYTE X Live-cell Imaging System

In this application note, we explore the use of live-cell imaging to determine proliferation of label-free cells as well as fluorescently labeled cells.
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In this technical note, we explore cell proliferation using the CELLCYTE XTM live-cell imaging system to conduct time-course experiments.

Cell Proliferation Using Live-cell Imaging: A Technical Note

In this technical note, we explore cell proliferation using the CELLCYTE XTM live-cell imaging system to conduct time-course experiments.
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We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.

Miniaturization and Automation of a full-length Single-Cell RNA-seq Workflow

We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.
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