3D Bioprinting Skin Models with Primary Dermal Fibroblast Cells
This application note demonstrates that a structurally complex and physically relevant in vitro skin model could be created using the BIO X 3D bioprinter, validating the suitability of the technology for skin tissue engineering. The full–thickness skin model was 3D bioprinted using primary dermal fibroblast cells embedded in GelXA SKIN bioink and keratinocytes embedded in ColMA. The bioprinted construct was cultured for a total of 14 days, at which point a robust skin tissue equivalent was observed.
Learn how to:
- 3D bioprint a full-thickness skin tissue model using the BIO X, GelXA SKIN, ColMA bioinks and primary dermal fibroblast cells.
- Facilitate the reorganization and proliferation of cells to form a more native cellular structure.
- Develop a robust platform for bioprinting epidermal and dermal layers that remain stable up to 14 days.
