3D Bioprinting Skin Tissue Models Using Primary Cells
This application note demonstrates that a structurally complex and physically relevant in vitro skin model could be created using the BIO X 3D bioprinter, validating the suitability of the technology for tissue engineering. The full–thickness skin 3D model was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink and keratinocytes embedded in ColMA. The bioprinted construct was cultured for a total of 14 days, at which point a robust skin tissue equivalent was observed.
Learn how to:
- 3D bioprint a full-thickness skin tissue model using the BIO X, GelXA SKIN, ColMA bioinks and primary dermal fibroblast cells.
- Facilitate the reorganization and proliferation of cells to form a more native cellular structure.
- Develop a robust platform for bioprinting epidermal and dermal layers that remain stable up to 14 days.
