A 3D Bioprinted Model of Multicellular Lung Cancer Assembloids

In this proof-of-concept study, the BIO X 3D bioprinter was used to produce multicellular lung cancer assembloid models. Cells were dispensed into a 24-well plate, and each well was filled with laminin-collagen-rich stromal environment that replicated the in vivo microenvironment or ECM. Using genetically encoded fluorescent cancer cells, the migration of cancer cells and merging of cancer spheroids within the matrix were visualized and further confirmed by histological analysis of fixed assembloids within the constructs. Immunofluorescent staining verified the expression of cell-surface and intracellular markers of 3D lung cancer tissue. These models could be easily adapted for other cancers and healthy tissue in developmental biology, regenerative medicine and toxicology applications.

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