A 3D Bioprinted Tumor Model for Immuno-oncology Applications
T cells play a critical role in achieving long-term immunity against infectious diseases as well as cancer cells. Historically, researchers studied T-cell mediated killing of cancer cells using 2D tumor models. But 2D models lack the biological relevance and complexity to accurately predict clinical outcomes. Therefore, to fabricate more physiologically relevant tumor models, more and more scientists now turn to bioengineering technologies, like the BIO X 3D bioprinter. This application note proposes an innovative protocol that leverages the droplet printing feature on the BIO X. Plus, this novel protocol for the automated biofabrication of murine tumor models is scalable for high-throughput preclinical trial screening during drug discovery.
Learn how to:
- Perform T cell cytotoxicity assays with the BIO X
- Droplet print murine lung cancer cells with collagen
- Culture tumor cells that remain confined and viable within a collagen matrix
- Test a T cell concentration-dependent increase in cytotoxicity
- Develop high-content imaging workflows customizable for other tumor models, including patient-derived xenografts
- Accelerate the drug screening process and drive clinical translation of personalized medicines
- Scale up high-throughput screening of immune checkpoint inhibitors and engineered T cells
