Application notes

Written by scientists for scientists, these papers cover a wide range of applications and highlight novel ways to optimize one’s research with our devices, technologies and consumables.

The long-distance transportation of mammalian cells usually requires cryo-preservation in dry ice or liquid nitrogen. Both of these methods are hazardous in nature and associated with high shipping costs. Here, we present the cost-effective shipping of 3D bioprinted constructs at ambient temperature using a novel encapsulation technology WellReady™ from Atelerix.

Room-temperature Transport of 3D Bioprinted Constructs Using WellReady™ In-plate Preservation

The long-distance transportation of mammalian cells usually requires cryo-preservation in dry ice or liquid nitrogen. Both of these methods are hazardous in nature and associated with high shipping costs. Here, we present the cost-effective shipping of 3D bioprinted constructs at ambient temperature using a novel encapsulation technology WellReady™ from Atelerix.
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Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations.

Printing Alginate Beads: A Technical Note

Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations.
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In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.

In Vitro 3D Lung Cancer Model Presents a More Relevant Expression of Junctional Proteins than 2D Cultures

In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.
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A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.

Advanced In Vitro 3D Models to Investigate iPSC Pluripotency and Capillary Network Formation of HUVECs

A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.
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In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.

3D Bioprinting Skin Tissue Models Using Primary Cells

In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.
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