More Relevant 3D Lung Cancer Models

More Relevant 3D Lung Cancer Models

To study the complexities of lung cancer, the leading cause of cancer-related deaths, and the mechanisms that drive metastasis, more physiologically relevant in vitro models are needed. We introduce a method to 3D bioprint in vitro lung models using A549 lung cancer cells embedded in gelatin methacrylate (GelMA) or Matrigel. After bioprinting with CELLINK’s BIO X™ and 14 days of cell culturing, we observed excellent tumor spheroid formation and physiologically relevant expression of β-catenin in 3D microenvironments. This lung disease model fabrication method can be used as a tool for automated assays in conjunction with high-throughput screening for drug development.

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Explore how well the A549 cells clustered and formed spheroids naturally when cultured in 3D versus the flat morphology of the single cells cultured in 2D.

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