To study the complexities of lung cancer, the leading cause of cancer-related deaths, and the mechanisms that drive metastasis, more physiologically relevant in vitro models are needed. We introduce a method to 3D bioprint in vitro lung models using A549 lung cancer cells embedded in gelatin methacrylate (GelMA) or Matrigel. After bioprinting with CELLINK’s BIO X™ and 14 days of cell culturing, we observed excellent tumor spheroid formation and physiologically relevant expression of β-catenin in 3D microenvironments. This lung disease model fabrication method can be used as a tool for automated assays in conjunction with high-throughput screening for drug development.
Explore how well the A549 cells clustered and formed spheroids naturally when cultured in 3D versus the flat morphology of the single cells cultured in 2D.