Collagen is found in over 30% of total body protein, making it an excellent biomaterial for tissue engineering and 3D printing applications. Collagen forms fibrous networks in the body, enhancing the structural integrity of the ECM while promoting cell adhesion, growth, biological signalling, and tissue morphogenesis.
Dissolve our Coll 1 sourced from high-quality rat tail tendon in the Reconstitution Agent A , neutralize, mix with cells, and build 3D cell culture constructs for tissue engineering or high-throughput screening. Once neutralized, Coll 1 solutions thermally crosslinks by self-assembly to form stable constructs. Use in combination with FRESH LifeSupport powder for support bath printing.
This product is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Coll 1 Lyophilizate is suitable for applications using a variety of cell lines including hepatocytes, fibroblasts and epithelial cells.
The Kit includes:
- 100 mg lyophilized Collagen type 1 (sterile)
- 30 ml Reconstitution Agent A (sterile)
- 5 ml Collagen Buffer (sterile)
- Consumables Kit C5 (Five clear cartridges, five luer lock adaptors, 15 nozzles, all sterile)
|Parameter, Testing, and Method||Coll 1 Lyophilizate #VL5000000010|
|Extraction Method||Acid – telocollagen|
|Package Size||100 mg|
|Storage Temperature of Collagen||2-10°C|
|Storage Temperature of Neutralization Solution||Room Temperature|
|Shelf Life||Minimum of 6 months from date of receipt|
|Collagen Purity – Silver Staining||>99%|
|pH||3.0-3.8 (in 0.02M Acetic Acid)|
|Kinetic Gel Test (Minutes)||<40|
|Gel Formation Tube Test (Minutes)||<40|
|Electrophoretic Pattern – Coomassie Blue||Characteristic|
|Sterility – USP modified||No growth|
|Endotoxin – LAL||<10.0 EU/mL|
|Osmolality (mOsmo H2O/kg)||<35|
|Cell Attachment Assay||Pass|
|Source||Rat Tail Tendon|
|Hydrogel Young’s Modulus E (Pa)||Characteristic|
Recommended for use with BIO X, a temperature-controlled printhead and a heated printbed. Not recommended for use with INKREDIBLE+ or INKREDIBLE.