Coll 1 Lyophilizate

3 490 kr4 790 kr

100 mg lyophilized collagen type 1. Type I collagen is one of the main structural proteins found in extracellular matrix (ECM) and connective tissue.

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Description

Collagen is found in over 30% of total body protein, making it an excellent biomaterial for tissue engineering and 3D printing applications. Collagen forms fibrous networks in the body, enhancing the structural integrity of the ECM while promoting cell adhesion, growth, biological signalling, and tissue morphogenesis.

Dissolve our Coll 1 sourced from high-quality rat tail tendon in the Reconstitution Agent A , neutralize, mix with cells, and build 3D cell culture constructs for tissue engineering or high-throughput screening. Once neutralized, Coll 1 solutions thermally crosslinks by self-assembly to form stable constructs. Use in combination with FRESH LifeSupport powder for support bath printing.

This product is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Coll 1 Lyophilizate is suitable for applications using a variety of cell lines including hepatocytes, fibroblasts and epithelial cells.

The Kit includes:

  • 100 mg lyophilized Collagen type 1 (sterile)
  • 30 ml Reconstitution Agent A (sterile)
  • 5 ml Collagen Buffer (sterile)
  • Consumables Kit C5 (Five clear cartridges, five luer lock adaptors, 15 nozzles, all sterile)

Specifications

Parameter, Testing, and Method Coll 1 Lyophilizate #VL5000000010
Sterilization Method Filtration
Extraction Method Acid – telocollagen
Form Solution
Package Size 100 mg
Storage Temperature of Collagen 2-10°C
Storage Temperature of Neutralization Solution Room Temperature
Shelf Life Minimum of 6 months from date of receipt
Collagen Purity – Silver Staining >99%
pH 3.0-3.8 (in 0.02M Acetic Acid)
Kinetic Gel Test (Minutes) <40
Gel Formation Tube Test (Minutes) <40
Electrophoretic Pattern – Coomassie Blue Characteristic
Sterility – USP modified No growth
Endotoxin – LAL <10.0 EU/mL
Osmolality (mOsmo H2O/kg) <35
Cell Attachment Assay Pass
Source Rat Tail Tendon
Hydrogel Young’s Modulus E (Pa) Characteristic

Recommended for use with BIO X, a temperature-controlled printhead and a heated printbed. Not recommended for use with INKREDIBLE+ or INKREDIBLE.

Additional information

Variant

Coll 1 Lyophilizate, 100 mg, Coll 1 Lyophilizate Kit

Resources

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