PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.
PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
|Parameter, Testing, and Method||PureCol® EZ Gel Type I Collagen #5074|
|Extraction Method||Enzyme – atelocollagen|
|Package Size||35 mL|
|Shelf Life||Minimum of 6 months from date of receipt|
|Collagen Concentration – Biuret||~5 mg/mL|
|Collagen Purity – Silver Staining||>99%|
|Kinetic Gel Test (Minutes)||<40|
|Gel Formation Tube Test (Minutes)||<40|
|Fibrillogenesis (Absorbance Units)||>0.5|
|Electrophoretic Pattern – Coomassie Blue||Characteristic|
|Sterility – USP modified||No growth|
|Endotoxin – LAL||<1.0 EU/mL|
|Osmolality (mOsmo H2O/kg)||300-360|
|Cell Attachment Assay||Pass|
|Hydrogel Young’s Modulus E (Pa)||Characteristic|
|L-Glutamine Source||Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine)|
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.