Description
Advanced BioMatrix offers PhotoCol®, a purified methacrylated type 1 bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.
The methacrylated type 1 collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content.
The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes.
Kit 1 – PhotoCol®
#5198-100MG
Item | Catalog Number | Package Size |
Methacrylated Collagen | #5198 | 100 mg |
PhotoCol® kits consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.
Kit 2 – PhotoCol®-IRG
#5201-1KIT
Item | Catalog Number | Package Size | Storage Temperature |
Methacrylated Collagen | #5198 | 100 mg | 2-10°C |
20 mM Acetic Acid | #5079 | 50 mL | 2-30°C |
Neutralization Solution | #5205 | 10 mL | Room Temperature |
Irgacure Photoinitiator | #5200 | 100 mg | 2-10°C |
A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.
The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.
Kit 3 – PhotoCol®-LAP
#5270-1KIT
Item | Catalog Number | Package Size | Storage Temperature |
Methacrylated Collagen | #5198 | 100 mg | 2-10°C |
20 mM Acetic Acid | #5079 | 50 mL | 2-30°C |
Neutralization Solution | #5205 | 10 mL | Room Temperature |
LAP Photoinitiator | #5269 | 100 mg | 2-8°C |
A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.
The photoinitiator consists of LAP to be formulated in 1X cell culture media or PBS which allows blue light photocrosslinking of the collagen at 405 nm.
To sterile filter, resuspend and filter through a 0.2 micron button filter.
Kit 4 – PhotoCol®-RUT
#5271-1KIT
Item | Catalog Number | Package Size | Storage Temperature |
Methacrylated Collagen | #5198 | 100 mg | 2-10°C |
20 mM Acetic Acid | #5079 | 50 mL | 2-30°C |
Neutralization Solution | #5205 | 10 mL | Room Temperature |
Ruthenium Photoinitiator | #5246 | 100 mg | Room Temperature |
Sodium Persulfate Photoinitiator | #5247 | 500 mg | Room Temperature |
A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.
The photoinitiator consists of Ruthenium and Sodium Persulfate to be formulated in 1x PBS or cell culture media which allows visible light photocrosslinking of the collagen at 400-450 nm. We recommend using antibiotics with the photoinitiator, or filtering through a 0.2 micron button filter for cell culture work.
Specifications
Parameter, Testing, and Method | Methacrylated Collagen #5198 |
Sterilization Method | Filtration |
Extraction Method | Acid – Telocollagen |
Form | Lyophilized Powder |
Degree of Methacrylation | >20% |
Package Size | 100 mg |
Shelf Life | Minimum of 6 months from date of receipt |
Shelf Life After Reconstitution | 2 Months |
Collagen Purity – Silver Staining | >99% |
Kinetic Gel Test (Minutes) | <40 |
Gel Formation Tube Test (Minutes) | < 40 |
Electrophoretic Pattern – Coomassie Blue | Characteristic |
Sterility – USP modified | No growth |
Endotoxin – LAL | <10.0 EU/mL |
Source | Bovine Hide |
Hydrogel Young’s Modulus E (Pa) | Characteristic |
Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.