PhotoCol® Methacrylated Type I Collagen

3 613 kr3 863 kr

PhotoCol® is 100 mg of lyophilized methacrylated Type I collagen. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness.

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Description

Advanced BioMatrix offers PhotoCol®, a purified methacrylated type 1 bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.

The methacrylated type 1 collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini.  > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content.

The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes.

 

Kit 1 – PhotoCol®

#5198-100MG

 Item  Catalog Number   Package Size 
 Methacrylated Collagen  #5198  100 mg

PhotoCol® kits consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.

Kit 2 – PhotoCol®-IRG

#5201-1KIT

 Item  Catalog   Number   Package   Size   Storage   Temperature 
 Methacrylated   Collagen  #5198  100 mg  2-10°C
  20 mM Acetic  Acid  #5079  50 mL  2-30°C
  Neutralization   Solution  #5205  10 mL  Room Temperature
  Irgacure   Photoinitiator  #5200  100 mg  2-10°C

 

A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.

The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.

 

Kit 3 – PhotoCol®-LAP

#5270-1KIT

 Item  Catalog   Number   Package   Size   Storage   Temperature 
 Methacrylated  Collagen  #5198  100 mg  2-10°C
 20 mM Acetic  Acid  #5079  50 mL  2-30°C
 Neutralization  Solution  #5205  10 mL  Room Temperature
 LAP  Photoinitiator  #5269  100 mg  2-8°C

 

A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.

The photoinitiator consists of LAP to be formulated in 1X cell culture media or PBS which allows blue light photocrosslinking of the collagen at 405 nm.

To sterile filter, resuspend and filter through a 0.2 micron button filter.

 

Kit 4 – PhotoCol®-RUT

#5271-1KIT

 Item   Catalog   Number   Package   Size   Storage   Temperature 
 Methacrylated   Collagen  #5198  100 mg  2-10°C
 20 mM Acetic Acid  #5079  50 mL  2-30°C
 Neutralization   Solution  #5205  10 mL  Room  Temperature
 Ruthenium   Photoinitiator  #5246  100 mg  Room  Temperature
 Sodium  Persulfate   Photoinitiator  #5247  500 mg  Room  Temperature

 

A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture.

The photoinitiator consists of Ruthenium and Sodium Persulfate to be formulated in 1x PBS or cell culture media which allows visible light photocrosslinking of the collagen at 400-450 nm. We recommend using antibiotics with the photoinitiator, or filtering through a 0.2 micron button filter for cell culture work.

 

Specifications

Parameter, Testing, and Method Methacrylated Collagen #5198
Sterilization Method Filtration
Extraction Method Acid – Telocollagen
Form Lyophilized Powder
Degree of Methacrylation >20%
Package Size 100 mg
Shelf Life Minimum of 6 months from date of receipt
Shelf Life After Reconstitution 2 Months
Collagen Purity – Silver Staining >99%
Kinetic Gel Test (Minutes) <40
Gel Formation Tube Test (Minutes) < 40
Electrophoretic Pattern – Coomassie Blue Characteristic
Sterility – USP modified No growth
Endotoxin – LAL <10.0 EU/mL
Source Bovine Hide
Hydrogel Young’s Modulus E (Pa) Characteristic

 

Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

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