Description
VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol sets the standard for purity (>99% collagen content), functionality and represents the only native-like human collagen offered. VitroCol human collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.
VitroCol is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. VitroCol is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2. VitroCol is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol human collagen is provided in user-friendly packaging for use and storage. VitroCol is sterile filtered and is supplied as a ready to use solution.
Specifications
| Parameter, Testing, and Method | VitroCol® Type I Collagen #5007 |
| Sterilization Method | Filtration |
| Extraction Method | Enzyme – atelocollagen |
| Form | Solution |
| Package Size | 20 mL, 100 mL |
| Storage Temperature | 2-10°C |
| Shelf Life | Minimum of 6 months from date of receipt |
| Collagen Concentration – Biuret | 2.9-3.2 mg/mL |
| Collagen Purity – Silver Staining | >99% |
| pH | 1.9-2.1 |
| Kinetic Gel Test (Minutes) | <40 |
| Gel Formation Tube Test (Minutes) | <40 |
| Fibrillogenesis (Absorbance Units) | >0.5 |
| Electrophoretic Pattern – Coomassie Blue | Characteristic |
| Sterility – USP modified | No growth |
| Endotoxin – LAL | <5.0 EU/mL |
| Osmolality (mOsmo H2O/kg) | <35 |
| Cell Attachment Assay | Pass |
| Source | Human Neo-Natal Fibroblasts |
| Hydrogel Young’s Modulus E (Pa) | Characteristic |
Product disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
