Polyethylene Glycol Diacrylate (PEGDA) hydrogels are powerful tools for uncovering basic cellular biology because they are considered biologically inert (“blank slate”) and their mechanical properties can be varied over a large range of moduli.
PEGDA is an emerging scaffold for tissue engineering and regenerative medicine since polymerization can occur rapidly at room temperature and requires low energy input, has high water content, is elastic, and can be customized to include a variety of biological molecules.
PEGDA is a crosslinker repeating polymer crosslinker and is >80% acrylated. It can be used as a thiol-reactive crosslinker in our HyStem systems at low concentration, or can be used to make a PEGDA-only hydrogel in the presence of a free-radical chain photoinitiator and light source. PEG-only gels do not typically support cell attachment without the incorporation of cellular attachment sites.
Store PEGDA in the original vial unopened at -20°C for up to one year. Reconstituted PEGDA solutions can be stored at -20°C for ~ one month.