Mebiol® Thermoreversible Hydrogel

3 490 kr

Mebiol® is a thermoreversible hydrogel that gels at 37°C and transitions back to a liquid at 4°C. Mebiol® is also biologically inert, allowing for easy spheroid formation in the hydrogel phase, and easy cell retrieval during the cold-melt phase.

SKU: #5180-10ML Category:


Mebiol® Thermoreversible Hydrogel is 1 gram of a copolymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG). The defining feature of Mebiol is its temperature reversible sol-gel transition. Chill Mebiol to turn it into a sol (meaning it handles like a liquid). Heat Mebiol to turn it into a solid hydrogel. The unique thermoreversible feature allows for extremely easy cell handling. Cultures are seeded into cooled Mebiol and recovered conveniently by cooling the culture vessel and centrifugation. In the gel state, the highly lipophilic and network structure of the Mebiol presents an efficient niche for cell proliferation, cell communication, gas and mass exchange, and protection of cells and tissue from shear forces.

Mebiol Thermoreversible Hydrogel is easy to use, non-toxic, 100% synthetic and has high transparency. Published applications include stem cell and pluripotent stem cell culture, expansion, and differentiation, spheroid culture, cell implantation, organ and tissue regeneration, drug delivery and more. Though Mebiol suppresses fibroblast growth, it has been commercialized as a cell/tissue culture reagent for ES cells, chondrocytes and cancer cells.

Parameter, Testing, and Method Mebiol Thermoreversible Hydrogel #5180
Package Size 10 mL
Form Lyophilized
Storage Temperatures Room temperature prior to solubilization. After solubilization, store at 2-8°C for 1 month or aliquot at -20°C for long term storage
Fluid Temperature 0-15°C
Gel Temperature > 25°C
Sterilization Method Ethylene Oxide
Shelf Life Minimum of 6 months from date of receipt