Mebiol® Thermoreversible Hydrogel is 1 gram of a copolymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG). The defining feature of Mebiol is its temperature reversible sol-gel transition. Chill Mebiol to turn it into a sol (meaning it handles like a liquid). Heat Mebiol to turn it into a solid hydrogel. The unique thermoreversible feature allows for extremely easy cell handling. Cultures are seeded into cooled Mebiol and recovered conveniently by cooling the culture vessel and centrifugation. In the gel state, the highly lipophilic and network structure of the Mebiol presents an efficient niche for cell proliferation, cell communication, gas and mass exchange, and protection of cells and tissue from shear forces.
Mebiol Thermoreversible Hydrogel is easy to use, non-toxic, 100% synthetic and has high transparency. Published applications include stem cell and pluripotent stem cell culture, expansion, and differentiation, spheroid culture, cell implantation, organ and tissue regeneration, drug delivery and more. Though Mebiol suppresses fibroblast growth, it has been commercialized as a cell/tissue culture reagent for ES cells, chondrocytes and cancer cells.
|Parameter, Testing, and Method||Mebiol Thermoreversible Hydrogel #5180|
|Package Size||10 mL|
|Storage Temperatures||Room temperature prior to solubilization. After solubilization, store at 2-8°C for 1 month or aliquot at -20°C for long term storage|
|Gel Temperature||> 25°C|
|Sterilization Method||Ethylene Oxide|
|Shelf Life||Minimum of 6 months from date of receipt|