Description
PhotoGel® 95% DS is a purified and lyophilized methacrylated gelatin for photocrosslinkable hydrogels. PhotoGel provides 3D tunable gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.
PhotoGel is produced from methacrylated gelatin where the gelatin has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. >75% of the total lysine residues of the gelatin molecule have been methacrylated.
Kit 1 – PhotoGel®
#5208-1GM
| Item | Catalog Number | Package Size | Storage Temperature |
| Methacrylated Gelatin | #5208 | 1 gram (2 x 500 mg) | -20°C |
Kit 2 – PhotoGel®-IRG
#5215-1KIT
| Item | Catalog Number | Package Size | Storage Temperature |
| Methacrylated Collagen | #5198 | 100 mg | 2-10°C |
| Irgacure Photoinitiator | #5200 | 100 mg | 2-10°C |
The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.
Kit 3 – PhotoGel®-LAP
#5272-1KIT
| Item | Catalog Number | Package Size | Storage Temperature |
| Methacrylated Collagen | #5198 | 100 mg | 2-10°C |
| LAP Photoinitiator | #5269 | 100 mg | 2-8°C |
The photoinitiator consists of LAP to be formulated in 1X PBS or cell culture media which allows blue light photocrosslinking of the collagen at 405 nm.
For aseptic work, we recommend filtering the solubilized LAP through a 0.2 micron button filter, as the photoinitiator does not come sterile.
Kit 4 – PhotoGel®-RUT
#5273-1KIT
| Item | Catalog Number | Package Size | Storage Temperature |
| Methacrylated Collagen | #5198 | 100 mg | 2-10°C |
| Ruthenium Photoinitiator | #5246 | 100 mg | Room Temperature |
| Sodium Persulfate Photoinitiator | #5247 | 500 mg | Room Temperature |
The photoinitiator consists of Ruthenium and Sodium Persulfate to be formulated in 1X cell culture media or PBS, which allows visible light photocrosslinking of the collagen at 400-450 nm.
For aseptic work, we recommend filtering the solubilized ruthenium and sodium persulfate through 0.2 micron button filters, as the photoinitiators do not come sterile.
Specifications
| Parameter, Testing, and Method | Methacrylated Gelatin #5208 |
| Sterilization Method | Filtration |
| Sterility – USP modified | No growth |
| Form | Lyophilized Powder |
| Package Size | 1 gram (2 x 500 mg) |
| Storage Temperature | -20°C |
| Shelf Life | Minimum of 6 months from date of receipt |
| Degree of Methacrylation | > 75% |
| Gelatin Source | Type A, 300 Bloom, Porcine |
| Hydrogel Young’s Modulus E (Pa) | Characteristic |
Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
