VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99% collagen content), functionality and represents the only native-like human collagen offered. VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.
VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved. VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. VitroCol®, lyophilized form is not recommend for the formation of a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is supplied as a sterile, lyophilized powder.
|Parameter, Testing, and Method||VitroCol® Lyophilized Type I Collagen #5008|
|Extraction Method||Enzyme – atelocollagen|
|Package Size||15 mg|
|Storage Temperature||-20°C prior to reconstitution and 2-10°C after reconstitution|
|Shelf Life||Minimum of 6 months from date of receipt|
|Shelf life after reconstitution||3 months|
|Collagen Purity – Silver Staining||>99.9%|
|Electrophoretic Pattern – Coomassie Blue||Characteristic|
|Sterility – USP modified||No growth|
|Endotoxin – LAL||<5.0 EU/mL|
|Cell Attachment Assay||Pass|
|Source||Human Neo-Natal Fibroblasts|
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.