Description
PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.
PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
Specifications
| Parameter, Testing, and Method | PureCol® EZ Gel Type I Collagen #5074 |
| Sterilization Method | Filtration |
| Extraction Method | Enzyme – atelocollagen |
| Form | Solution |
| Package Size | 35 mL |
| Storage Temperature | 2-10°C |
| Shelf Life | Minimum of 6 months from date of receipt |
| Collagen Concentration – Biuret | ~5 mg/mL |
| Collagen Purity – Silver Staining | >99% |
| pH | 6.9-7.4 |
| Kinetic Gel Test (Minutes) | <40 |
| Gel Formation Tube Test (Minutes) | <40 |
| Fibrillogenesis (Absorbance Units) | >0.5 |
| Electrophoretic Pattern – Coomassie Blue | Characteristic |
| Sterility – USP modified | No growth |
| Endotoxin – LAL | <1.0 EU/mL |
| Osmolality (mOsmo H2O/kg) | 300-360 |
| Cell Attachment Assay | Pass |
| Source | Bovine Hide |
| Hydrogel Young’s Modulus E (Pa) | Characteristic |
| Medium Supplement | DMEM/F-12 |
| L-Glutamine Source | Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine) |
Product disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
