Description
PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.
PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
Specifications
| Parameter, Testing, and Method | PureCol® EZ Gel Type I Collagen #5074 |
| Sterilization Method | Filtration |
| Extraction Method | Enzyme – atelocollagen |
| Form | Solution |
| Package Size | 35 mL |
| Storage Temperature | 2-10°C |
| Shelf Life | Minimum of 6 months from date of receipt |
| Collagen Concentration – Biuret | ~5 mg/mL |
| Collagen Purity – Silver Staining | >99% |
| pH | 6.9-7.4 |
| Kinetic Gel Test (Minutes) | <40 |
| Gel Formation Tube Test (Minutes) | <40 |
| Fibrillogenesis (Absorbance Units) | >0.5 |
| Electrophoretic Pattern – Coomassie Blue | Characteristic |
| Sterility – USP modified | No growth |
| Endotoxin – LAL | <1.0 EU/mL |
| Osmolality (mOsmo H2O/kg) | 300-360 |
| Cell Attachment Assay | Pass |
| Source | Bovine Hide |
| Hydrogel Young’s Modulus E (Pa) | Characteristic |
| Medium Supplement | DMEM/F-12 |
| L-Glutamine Source | Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine) |
Product disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Not available for shipment to Europe.
