Description
This Type IV Collagen is isolated from human placenta and is purified using a multi-step process. The product is supplied as a sterile, lyophilized powder containing 5 mg of Type IV collagen per vial.
Type IV Collagen is the primary collagen found in the extracellular basement membranes separating a variety of epithelial and endothelial cells. It is a major component of the dermal-epidermal junction where it is mostly found in the lamina densa. It is a heterotrimeric molecule containing two Alpha 1-like and one Alpha 2-like chains.
Type IV Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type IV collagen may be used to culture epithelial, endothelial, muscle, nerve and many other cell types. Additionally, this product is suitable for use as a substrate for collagenase assays and positive controls.
Specifications
| Parameter, Testing, and Method | Type IV Collagen #5022 |
| Sterilization Method | Irradiation |
| Form | Lyophilized Powder |
| Package Size | 5 mg |
| Storage Temperature | -20°C prior to reconstitution and 2-10°C after reconstitution |
| Shelf Life | Minimum of 6 months from date of receipt |
| Shelf Life after Reconstitution | 3 months |
| Adventitious Agents | Human source has been tested and found negative for Hepatitis B, C and immunodeficiency virus-1. |
| Electrophoretic Pattern – Coomassie Blue | Characteristic |
| Sterility – USP modified | No growth |
| Endotoxin – LAL | <1.0 EU/mL |
| Cell Attachment Assay | Pass |
| Source | Human Placenta |
Product disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
