BIO-CELLX---Biodispenser

BIO CELLX

Automated 3D Cell Culture. Simplified.

Introducing Biodispensing

As industry leaders in bioprinting, and thanks to our valuable customers, our knowledge of 3D biology research remains unrivaled. This in-depth understanding of 3D biology and our customers’ needs has enabled the development of biodispensing. Through a lens of bio convergence, we have harmonized the best practices within bioprinting with the higher throughput, automation-capable technology present within the liquid handling field. Now we’re introducing the world’s first ever biodispenser – and a new modality to the world of biofabrication. A hassle- and engineering-free method to develop reproducible 3D models which are compatible with existing analysis workflows.

Welcome to the era of biodispensing.

Effortless-Hydrogel-Extrusion-White

Tuned for hydrogel dispensing

Each of the three material mixing stations in the BIO CELLX offer precise temperature control, ranging from 0°C to 60°C, allowing for hassle-free printing of ECM Hydrogels

Well-White

Cell-friendly automation

The BIO CELLX eliminates tedious steps associated with bioink and cell with a patented cell and bioink mixing procedure grounded in maximizing cell viability. Providing users with walk-away capabilities like never before.

Unprecedented-Reproducibility-White

Unprecedented reproducibility

Using positive displacement technology, and features like onboard cell mixing, the BIO CELLX provides unprecedented reproducibility across wells and constructs

Workflow

The BIO CELLX enables our customers a cost-effective and time-efficient automated 3D cell culture workflow. The easy-to-use biodispensing system allows you the time and energy to focus on your research.

Preparation
Culture cells
Culture cells of your choice
Select a bioink
Select a material for which the CELLINK team has developed validated dispensing protocols, or use another material and develop your own dispensing protocol with Discovery Mode.
BIO CELLX
Dispensing protocol
Bioink & Cells Mixing
BIO CELLX automatically prepares the bioink and mixes it with cell suspension
Dispensing
BIO CELLX dispenses 3D models using pre-set parameters at a press of a button
Crosslinking
BIO CELLX crosslinks the models using built-in photocrosslinking and thermal modules
Analysis
Post dispensing
Add cell medium, incubate, and dose drugs to your 3D models
Assays and analyses
Analyze your 3D models using standard assays or imaging methods
See the technology that powers high-throughput 3D Cell Culture

Delve inside the BIO CELLX

Features

Precise & reproducible

A high-precision positive displacement extrusion system which provides reproducible results every single time, regardless of the dispensed material.

Uncompromised sterility

CELLINK’s fully enclosed, proprietary Clean Chamber technology, combined with a patent pending de-lidding solution, ensures the sterility of the entire workflow when working on a benchtop in a laboratory environment.

Built-in dispensing protocols

Save time with easy-to-use dispensing protocols
containing validated parameters for 3D model
fabrication, with cell-line flexibility.

Automated Calibration

To achieve an industry-leading positional accuracy, a sensor suite measures the position of each of the dispensing orifices in relation to the position of the vessel. An algorithm compensates any inaccuracies to ensure the precise position of the dispensed model.

Optimized for high cell viability

Every process within the BIO CELLX was developed through a lense of maximizing cell viability, ensuring highly functional cellular models.

No modeling required

Thanks to the variety of 3D models built into DNA Studio ACE there’s no longer a need to spend time modeling in CAD software.

FEATURE HIGHLIGHT: Proprietary Material Mixing Station

Effortless biomaterial preparation. Every time.

With a novel patent pending dispensing mechanism onboard, the BIO CELLX brings a new degree of ease to creating physiologically relevant 3D models, with the ideal matrix environment, and biomechanical cues for your downstream assays.

Maximize workflow efficiency

Thanks to the automatic mixing of bioinks, reagents and cell suspension, the BIO CELLX significantly reduces the preparation time in your 3D cell culture process.

Unparalleled repeatability

Automated bioink preparation reduces the variation in mechanical properties, cell viability, pH and air content of samples, enabling consistent results every single time.

Homogenous cell density

With gentle mixing built into the BIO CELLX, cell density remains evenly distributed throughout the dispensing process, ensuring reproducible 3D models across wells.

3D geometries to accelerate your discoveries

Select a construct to see relevant assays

Or see the full table of assays here

Droplet
Droplet

Droplet is recommended for

Immuno-oncology model
Tissue model
Spheroid
Organoid
Complex organoid / tumoroid model
Droplet-in-Droplet
Droplet-in-Droplet

Droplet-in-Droplet is recommended for

Spheroid
Organoid
Cell-invasion
Chemo-attraction
Metastasis model
Immuno-oncology model
Tissue model
Complex organoid / tumoroid model
Triplet Droplet-in-Droplet
Triplet Droplet-in-Droplet

Triplet Droplet-in-Droplet is recommended for

Spheroid
Organoid
Cell-invasion
Chemo-attraction
Metastasis model
Immuno-oncology model
Tissue model
Complex organoid / tumoroid model
Line
Line

Line is recommended for

Spheroid
Organoid
Immuno-oncology model
Tissue model
Complex organoid / tumoroid model
Line-by-line
Line-by-line

Line-by-line is recommended for

Spheroid
Organoid
Cell-invasion
Chemo-attraction
Metastasis model
Immuno-oncology
Tissue model
Complex organoid / tumoroid model
Triple Line-by-line
Triple Line-by-line

Triple Line-by-line is recommended for

Spheroid
Organoid
Cell-invasion
Chemo-attraction
Metastasis model
Immuno-oncology model
Tissue model
Complex organoid / tumoroid model
Grid
Grid

Grid is recommended for

Spheroid
Organoid
Cell-invasion
Chemo-attraction
Metastasis model
Immuno-oncology model
Tissue model
Multilayer tissue model
Complex organoid / tumoroid model
Hydrogel-covered droplet array
Hydrogel-covered droplet array

Hydrogel-covered droplet array is recommended for

Spheroid
Organoid
Cell-invasion
Chemo-attraction
Metastasis model
Immuno-oncology model
Tissue model
Complex organoid / tumoroid model

Areas of Impact

Drug Discovery

Expedite the identification of high impact drug candidates.

More Details →​
Stem Cell Biology

Advance the potential of stem cells in 3D.

More Details →​
Cancer Research

Mimic the in-vivo tumor microenvironment with our constructs.

More Details →​
Regenerative Biology

Ascertain the regenerative potential of tissues with our constructs.

More Details →​
Personalized Medicine

Develop patient-specific models with unprecedented ease.

More Details →​
Tissue Modeling

Simulate tissue-specific structure with multiple cell types.

More Details →​
Disease Modeling

Unleash new and improved therapies and diagnostics.

More Details →​

&
many more.

Software

DNA Studio ACE

Maximize walk-away capabilities through the power of automation. A brand-new intuitive version of DNA Studio guides users through the entire biodispensing process, from setting up the system to selecting one of the pre-set and pre-validated protocols.

No training required.

Key features:

BIO CELLX Studio On Tablet
Enhanced flexibility when required

Discovery mode

We understand research needs to be developed over time and, while our protocols are continuously validated by our scientists, Discovery Mode allows you the ability to develop your own custom protocols.
Optimize dispensing parameters to work with hydrogels you know.
Custom bioink support

Optimize the dispensing parameters to work with hydrogels that you already know.

Optimize mixing parameters

Adjust mixing parameters to achieve higher cell viability or better homogeneity of the solution.

STL-file support

3D bioprint from STL-files for full freedom of design

Flexible material choice

Dispense up to 3 biomaterials and assign them per well or construct

Technical specifications

Outer dimensions (L x W x H)

765 x 580 x 555 mm

Build volume
125 x 85 x 38​ mm
Build surface compatibility​
Multi-well plates 6 to 96 wells
Theoretical resolution XY
3 µm
Theoretical layer resolution
1.5 µm
Material mixing stations
3
Bioink max volume
3.0 mL
Cell media max volume
2.0 mL
Source fluid temperature range
0-60 °C
Volume unit step
0.1​ µL​
Theoretical minimum droplet size
5 µL​
Printbed temperature range​
0-60​ °C
Photocuring system
365 nm​, 405 nm​, 485 nm​, 520​ nm​
Filter class, chamber flow
HEPA 14
UV sterilization
UV-C (275 nm)
Calibration​
Automatic
User interface​
Tablet​
Connectivity
Ethernet

Interested? Contact us.

Every customer is our partner. Through available and excellent support, our bioprinting application specialists will help you achieve great results.