Working side-by-side with our collaborators, we understand exactly what they need to achieve groundbreaking results. In that effort, we work continuously to develop new tissue-specific bioinks and bioprinting models that will make an immediate positive impact on the research field.
Today, we would like to share our recent in-house research focused on developing our new Skin Tissue Model Kits!
Figure 1. Elastin expression in native skin. Elastin appears in red (1:50 dilution), and nuclei stained with DAPI appear in blue (1:50 dilution). Secondary antibody: Alexa Fluor 488.
What is it?
The full bioprinting experience of skin tissue models is impossible without two things: 1) bioprinting of physiologically functional tissue; 2) comprehensive tissue analysis using specific antibodies. The first challenge can be addressed by the bioinks that are explicitly developed to support maturation and proliferation of both the thick dermis layer and the stratified epidermis of a skin tissue (Figure 2). The second challenge simply vanishes if you have deliberately optimized antibodies that illustrate cell phenotypes in the created skin models.
Choose between a CELLINK or GelXA base.
The end user can choose either CELLINK SKIN or GelXA SKIN to build their own skin tissue models. Both bioinks promote cell proliferation, motility and differentiation within the different compartments of the skin tissue model, and provide advantageous bioprinting characteristics. For instance, GelXA SKIN bioink provides the beneficial biological properties of GelMA, minimal background interference in both fluorescence observations and histochemistry analysis and the possibility to control the mechanical properties of the tissue through ionic and photoinduced crosslinking methods. Meanwhile, CELLINK SKIN provides smooth printing with high precision at a wide temperature range and longer storage time as it is completely insensitive to light.
Includes the antibodies you need for an accurate analysis
The antibodies provided within the Skin Tissue Model Kits are selective for key factors in a native skin tissue, including collagen type 1, keratin 10 and elastin. Collagen type 1 is produced by dermal fibroblasts and is the main component in the dermis. It is responsible for skin functionality, structure and firmness. Using the specific antibody included in the Kits, the detection of collagen type 1 becomes a straightforward task (Figure 2A). Keratin 10 is an important early proliferation factor of keratinocytes, mostly those closest to the dermis. Its expression is clearly seen after treatment with the antibody provided in the kits (Figure 2B). Finally, elastin is the extracellular protein responsible for the elasticity of the skin; it is present in the dermis in close connection with other extracellular matrix proteins such as collagens. Figure 2C shows the expression of elastin obtained with the help of the antibody in the Kits. The Skin Tissue Model Kits are compatible with the other types of skin cells of your choice such as melanocytes for creation of a photo-sensitive skin tissue model, microvascular endothelial cells supplemented with the VasKit for vascularized skin tissue model or even cells from donors to make patient-specific tissue models.
Figure 2. A) Collagen type 1 expression in specially treated skin tissue model created with GelXA SKIN bioink. Collagen type 1 appears in green (1:50 dilution), and nuclei stained with DAPI appear in blue (1:50 dilution). B) Keratin 10 expression in skin tissue model with keratinocytes embedded into GelXA SKIN bioink. Keratin 10 appears in magenta (1:200 dilution), and nuclei stained with DAPI appear in blue (1:50 dilution). C) Elastin expression in skin tissue model with keratinocytes embedded into CELLINK SKIN bioink. Elastin appears in red (1:50 dilution), and nuclei stained with DAPI appear in blue (1:50 dilution). Secondary antibody: Alexa Fluor 488. Scale bar 100 µm.