Rapid Fabrication of Ready-to-Use Gelatin Scaffolds with Prevascular Networks Using Alginate Hollow Fibers as Sacrificial Templates

In this study, we developed a facile manufacturing method for interconnected prevascular networks using calcium chloride (CaCl2) cross-linked alginate hollow fibers as sacrificial templates. The resulting network can be used to deliver oxygen and nutrients and remove waste for embedded cells in large-volume gelatin scaffolds during in vitro culturing. The sacrificial templates were printed by customized coaxial nozzles and embedded in scaffolds made of a mixture of gelatin, microbial transglutaminase (mTG), and sodium citrate. During the cross-linking of gelatin and mTG, the sacrificial templates started to dissolve from the scaffold–template interface due to the presence of the sodium citrate in the gelatin.

 

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