EPI X for printing intestinal cells

The human intestine plays a critical role in absorbing not just water and nutrients, but also pharmaceutical drugs. In addition to its primary function, it acts as a protective barrier against pathogens. 3D printing intestinal cells overcomes the limitations of traditional 2D cultures, enabling more advanced and specific tissue-like structures. To meet the needs of your research, 3D-printed constructs can be cultured in filter inserts to create a stable platform for studying nutrient transport and bioactive molecules, pharmaceutical effects and intestinal barrier function.


The aim of this project was to evaluate the viability of Caco 2 cells 3D bioprinted in CELLINK EPI X ink using the CELLINK BIO X.


CELLINK products used

  • BIO X.

Caco 2, Bright-field, 10x magnification

Cell Information

Caco2 is a cell line derived from human epithelial colorectal adenocarcinoma and it is used widely as a model of the intestinal-epithelial barrier.

This cell line has previously been used to study if components of the sigma B regulon, which controls the transcriptional response to stress, in Listeria monocytogenes contribute to cell invasion. It has also been used to study the effect of maslinic acid, a promising chemopreventive agent, on colon cancer cell lines.

Cells can be printed in monoculture or in coculture, in separate layers or compartments. Use our BIO X bioprinter and print up to three different types in one go!

Printing Data

Caco2 cells were mixed with CELLINK EPI X bioink in 1:10 ratio and discs were printed with the BIO X.


  • Cell type: Caco2
  • Bioink: CELLINK EPI X
  • Concentration: 10 million cells /mL of bioink
  • 3D bioprinter: BIO X
  • 3D model: disc 3 x 0.6 mm
  • Nozzle diameter/gauge: 0.410 mm (22 G)
  • Speed: 10 mm/s
  • Pressure: 3-5 kPa


Cell number and viability in 3D-bioprinted constructs remained stable between Days 1 and 8. All analysed samples exhibited over 80% viability. Stable cell number and high viability indicate the beneficial effect of the CELLINK EPI X bioink, which is demonstrated to foster a suitable environment for cell growth and proliferation.