Working side-by-side with our collaborators, we understand exactly what they need to achieve groundbreaking results. In that effort, we work continuously to develop new tissue-specific bioinks and bioprinting models that will make an immediate positive impact on the research field.
Today, we would like to share our recent in-house research focused on developing our new Liver Tissue Model Kits!
Figure 1. Collagen type 1 production in co-cultured HepG2 and LX2 in (A) GelXA LAMININK 111 and (B) CELLINK LAMININK 111 at day 7 of culture. Collagen type 1 expression appears in green (1:50 dilution), and nuclei stained with DAPI appear in blue (1:50 dilution). Secondary antibody: Alexa Fluor 488 conjugated.Scale bar 100 µm.
What is it?
CELLINK’s Liver Tissue Model Kits provides you with all the tools necessary to generate bioprinted liver tissue models and perform a complete analysis (Figure 1). Such liver models maintain a physiological 3D environment that resembles in vivo conditions, which enhances liver cell processes. As a result, modeled liver tissues demonstrate native-like uptake and dose responses, thus enabling, for instance, relevant drug screening. The complexity of the bioinks in the Liver Tissue Model Kits helps you to tailor the liver models for specific investigations of many different cell types including hepatocytes, stellate cells, Kupffer cells and endothelial cells. Meanwhile, optimized antibodies included in the Liver Tissue Model Kits allow for direct analysis of the cells within the constructs on selective key markers for liver functionality.
Choose between a CELLINK or GelXA base.
CELLINK- or GelXA-based bioinks functionalized with laminin 111 are provided in the Kits; they are the essentials for 3D bioprinting the models. The user can determine the optimal bioink for the target cell types as a single or multi-cell model. The GelXA LAMININK 111 provides the biological properties of GelMA with printability at a wide temperature range, including room temperature. It has dual ionic and photoinduced crosslinking capabilities to better accommodate cellular sensitivity, allowing you to tune mechanical characteristics of the models to be more tissue specific. The bioink has minimal optical interference in both bright-field and fluorescence observation for nondestructive analysis. In histochemistry analysis, GelXA LAMININK 111 has minimal background effects, enabling better investigation of cell-cell and cell-matrix interactions. CELLINK LAMININK 111 is also advantageous with stable and versatile printability characteristics at any temperature, in addition to not being sensitive to light. The nanocellulose in the CELLINK bioinks provides a fibrillar network that can influence the interconnections of the different liver cell types, for example, potentially facilitating the formation of a bile canalicular network or the alignment of stellate cells within a co-culture tissue model.
Includes the antibodies that you need for an accurate analysis.
Direct functional analysis of the liver tissue model can be conducted with histological analysis through immunofluorescent imaging or immunohistochemistry. The collagen type 1 antibody is to evaluate the production of collagen type 1 by the stellate cells in bioprinted co-culture constructs (Figure 1), while the ABCC2 antibody is to evaluate the transporter multidrug resistance protein 2 (MRP2) in hepatocytes (Figure 2 A-B). Moreover, the antibodies included in the Liver Tissue Model Kits are intended for the single or co-culture incorporation of hepatocytes and stellate cells in bioprinted models (Figure 2 C-D).
Figure 2. The ABCC2 (MRP2) antibody staining of hepatocyte HepG2 in co-cultured constructs bioprinted with (A) GelXA LAMININK 111 and (B) CELLINK LAMININK 111. ABCC2 expression appears in green (1:200 dilution), and nuclei stained with DAPI appear in blue (1:50 dilution). C) The CYP3A43 antibody staining of primary hepatocytes in C) clusters and D) single cells. CYP3A43 expression appears in green (1:1000 dilution), and nuclei stained with DAPI appear in blue (1:50 dilution). Secondary antibody: Alexa Fluor 488 conjugated. Scale bar 100 µm.