Streamlined Workflow for CRISPR-edited Stem Cell Clones

While CRISPR-Cas9-mediated gene editing can enhance the success rate of stem cell therapies, the selection and single cell cloning in high-throughput cell line development often requires multiple tedious steps. We introduced a streamlined workflow using the F.SIGHT™ single-cell dispenser, which enables gentle, high-throughput screening and isolation by dispensing cells encapsulated in single droplets.

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Explore how the highly sensitive F.SIGHT was able to detect even low fluorescent signals for the cell sorting of isolated genome-edited RANKL knockout MSC clones with an efficiency of >93%, while retaining osteogenic differentiation potential.

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