Streamlined Workflow for CRISPR-edited Stem Cell Clones
While CRISPR-Cas9-mediated gene editing can enhance the success rate of stem cell therapies, the selection and single cell cloning in high-throughput cell line development often requires multiple tedious steps. We introduced a streamlined workflow using the F.SIGHT™ single-cell dispenser, which enables gentle, high-throughput screening and isolation by dispensing cells encapsulated in single droplets.
Explore how the highly sensitive F.SIGHT was able to detect even low fluorescent signals for the cell sorting of isolated genome-edited RANKL knockout MSC clones with an efficiency of >93%, while retaining osteogenic differentiation potential.
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