BIO X™

The go-to 3D bioprinter for cell biologists, tissue engineers and biomedical researchers.

Unparalleled bioprinting experience

Explore the most user-friendly and flexible bioprinter in the world.

Unparalleled bioprinting experience

Explore the most user-friendly and flexible bioprinter in the world.

Limitless possibilities

BIO X is the first 3D bioprinter in the world with Intelligent Printheads (iPH), ensuring your research is always at the forefront.

Limitless possibilities

BIO X is the first 3D bioprinter in the world with Intelligent Printheads (iPH), ensuring your research is always at the forefront.

With an ever-growing list of Intelligent Printheads, the possibilities are limitless, giving you more freedom to push your research further.

Designed to Do More

The BIO X’s exceptional features maximize versatility to enhance your success in the following application areas and beyond.

Advanced Functionality and Versatility

The BIO X’s compatibility with virtually any material makes it the bioprinter of choice for industry leaders at the forefront of today’s biggest scientific breakthroughs. Whether you are automating 3D cell cultures, developing complex tissue constructs or testing new drug compounds, the BIO X has the advanced functionality and versatility to streamline workflows in a wide range of application areas.

Push the Limits

Bioprint with a wider range of materials and cell types thanks to precise printhead and printbed temperature control.

Push the Limits

Bioprint with a wider range of materials and cell types thanks to precise printhead and printbed temperature control.

With a printhead temperature range of 4°C to 250°C and a printbed temperature range between 4°C and 65°C, enjoy precise control of temperatures when working with temperature-sensitive materials like collagen and gelatin. The possibilities are limitless when you are able to work with a wider range of materials and cell types.

Enhanced Precision

The BIO X comes standard with motors of a 1 µm resolution, ensuring you can control the finest movements.

EXPLORE BIO X6™

The next generation bioprinter with 6 printheads allows the use of several crosslinking modalitites and multimaterial constructs simultaneously, enabling even more complex models.

Universal Compatibility

The BIO X’s open material platform is designed to give you the ability to bioprint with the widest range of materials.

Universal Compatibility

The BIO X’s open material platform is designed to give you the ability to bioprint with the widest range of materials.

We offer an extensive portfolio of bioinks but understand the importance of material development, which is why we built the BIO X to be an open system that is compatible with the widest range of materials available. Start developing your own bioinks with our high-quality bioink development products or explore our ready-to-print bioinks.

Patented Clean Chamber for Better Cell Safety

Built-in UV sterilization and a HEPA filter bring the sterility of the biosafety cabinet to the benchtop.

Clean Chamber for Better Cell Viability

Built-in UV sterilization and HEPA filters bring the sterility of the biosafety cabinet to the benchtop.

Dual high-powered fans channel air through a HEPA H14 filter to remove 99.995% of unwanted particles and microorganisms as part of the Clean Chamber™ technology, which also includes UV-C germicidal lights, rounded edges and positive air pressure. Initiate Clean Chamber before beginning your experiment to ensure full sterility around the print area for the duration of the print.

User-centered Design

Auto calibrate the printer directly from the touchscreen for added efficiency, even while wearing gloves.

User-centered Design

Auto calibrate the printer directly from the touchscreen for added efficiency.

Maximum Convenience

With onboard or desktop software options, a built-in compressor and auto calibration, the BIO X is ready to use right out the box.

Maximum Convenience

Onboard or desktop software options, built-in compressor and auto calibration.

Application Notes

Written by scientists for scientists, these papers highlight novel ways to optimize one’s research with the BIO X Bioprinter.

Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations.

Printing Alginate Beads: A Technical Note

Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations.
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In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.

In Vitro 3D Lung Cancer Model Presents a More Relevant Expression of Junctional Proteins than 2D Cultures (English)

In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.
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A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.

Advanced In Vitro 3D Models to Investigate iPSC Pluripotency and Capillary Network Formation of HUVECs (English)

A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.
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In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.

3D Bioprinting Skin Tissue Models Using Primary Cells

In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.
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Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X.

Evaluating Liver Toxicity in Bioprinted Mini Livers

Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X.
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Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models.

Comparing Drug Response in 2D Cultures and 3D Bioprinted Tumoroids

Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models.
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3D bioprinting a syngeneic tumor model of murine lung cancer on the BIO X to evaluate an immune checkpoint inhibitor (PD-1) in a 3D T cell cytotoxicity assay.

3D Bioprinted Tumor Model for Immuno-oncology Applications

3D bioprinting a syngeneic tumor model of murine lung cancer on the BIO X to evaluate an immune checkpoint inhibitor (PD-1) in a 3D T cell cytotoxicity assay.
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Technical Data 
Outer dimensions (L*W*H), mm  477x441x365 
Weight, kg 18
Build Volume, mm 
130 x 90 x 70 mm
Build surface compatibility  Multi-well plates, petridishes, glass slides 
Resolution XY, μm  1 
Layer Resolution, μm   1 
Pressure range (internal pump), kPa  0-200 
Pressure range (external air supply), kPa  0-700 
No. of printhead slots  3 
Photocuring sources (built-in), nm  365, 405 
Printbed temperature range, °C  4-65
Printhead temperature range, °C  4-250 (printhead specific) 
Filter class, chamber air-flow  HEPA 14 
UV-sterilization  UV-C (275nm), 20mW output 
Calibration Options  Manual and Automatic  
User Interface  Integrated Display, DNA Studio 
DNA Studio OS compatibility  Windows, Mac OS 
Connectivity  USB Storage, Ethernet connection 
Supported file formats, software  .gcode, .stl

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