Synthetic Biology

Accelerating complex synthesis workflows

Introduction

Synthetic biology describes the engineering or re-designing of biological systems. Metabolic engineering or the construction of artificial gene networks and signaling pathways enable the re-programming of natural mechanisms for new purposes and constructing synthetic organisms with desired properties. Synthetic biology approaches have provided solutions for the production of chemicals used in medicine, manufacturing and agriculture.

Challenging tasks require powerful tools

The main challenge in synthetic biology is to abstract the complexity of biological regulatory networks into biological parts with well-defined functions. These parts can be subsequently assembled in a modular fashion to yield a huge variety of new functionalities.

meeting the needs of complex synthetic biology workflows

Precise handling and concentration control of DNA is essential in assembling sequence libraries from functional parts. The I-DOT technology allows highly multiplexed low-volume dispensing of DNA fragments and reagents in a contact-free manner with no cross-contamination. Multiple liquids can be rapidly distributed and assembled, meeting the needs of complex synthetic biology workflows. Traditional synthesis reactions can be scaled down by orders of magnitude, saving reagent costs and time. The I-DOT is compatible with a wide range of plate-handling robots and can be integrated into fully automated platforms.

Model organisms to screeen synthetic parts

In addition to rational design approaches and cell-free screening systems, microbial model organisms, such as E. coli or S. cerevisiae, are used for developing and testing new synthetic parts. The well-characterized genomes and the availability of easy methods for genetic manipulation in these organisms combined with high reproduction rates make them valuable for the screening of sequence libraries.

Automated single-cell cloning

The b.sight™ single-cell dispensing technology provides simplified single-cell cloning workflows for bacteria, yeast and other small fungi. The b.sight enables direct, label-free and automated isolation of single cells after transformation. Dispensing the cells into liquid medium replaces manual and time-consuming steps such as streaking on agar and colony picking.

Related publications

Cell-Free Prototyping of AND-Logic Gates Based on Heterogeneous RNA Activators

Author(s): François-Xavier Lehr, Maleen Hanst, Marc Vogel, Jennifer Kremer, H. Ulrich Göringer, Beatrix Suess, and Heinz Koeppl

Technische Universität Darmstadt

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I-DOT™

A premier non contact liquid handling solution designed to provide efficiency and speed to your dispensing processes. The I-DOT can assist in miniaturizing your assays, compound screening or sample prep.

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b.sight™

Dispense single bacteria using proven single-cell dispensing technology. Enables prokaryotic cell isolation in the sub-micron range. The high-resolution optics makes even the smallest cells visible.