Understanding cell physiology in the presence of pharmaceutical reagents
Cell-based assays play a pivotal role in understanding cell physiology in the presence of pharmaceutical reagents in both healthy and diseased states and have been an important pillar of drug discovery. Frequently, cell-based assays are used in high throughput screening (HTS) of compound libraries. New assay platforms are evolving rapidly to address increasingly complex biological challenges—mimicking disease biology, preserving signaling pathways, modelling drug responses and recreating environments conducive to tissue development. Such assays are based on traditional cell culture methods with the possibility of measuring various functional activities like proliferation, toxicity, changes in morphology or gene expression.
While the majority of available assays are based on traditional 2D cell culture, 3D cell culture systems are increasingly adopted to reduce large-scale and high-cost animal testing. At CELLINK, we provide innovative technologies to simplify cell-based assays and increase automation. In addition, as the world leader in bioprinting, we are able to offer a variety of technologies that help our customers transition from traditional 2D cell culture to 3D systems.
Multiplexed low-volume dispensing for high-throughput screening
High-throughput screening (HTS) of compound libraries, reagent compositions or drug concentrations requires accurate and fast liquid handling technology. Our I-DOT non-contact low-volume dispensing technology was designed to address the most complex liquid-handling tasks with unrivaled speed. The system can readily dispense into standard microwell plates including 1536 and higher density formats. Low-volume dispensing down to the single-digit nanoliter range, no need for pipette tips and best-in-class precision helps you save reagent and consumable costs, increase reproducibility and raise the Z prime value of your assay.
Precise and gentle cell dispensing
Key for automating cell-based assays is an equal distribution of cells into hundreds or thousands of microwells. Mammalian cells often tend to sediment in dispensing nozzles or pipette tips. Our I-DOT and I-DOT Mini instruments allow for fast and gentle cell dispensing without impacting viability. Common applications include the distribution of adherent cells into culture well plates or dispensing cancer cells for tumor spheroid formation.
MORE PHYSIOLOGICALLY RELEVANT MODELS
Traditional assays are based on cultivating cells in a 2D monolayer. Despite the advantages with respect to high-throughput screening and automation, these systems often do not reflect all relevant aspects that cells experience in vivo. At CELLINK, we offer entire solutions for building and analyzing more physiologically relevant 3D tissue models with our bioprinters and ready-to-use bioinks. Our team of bioprinting experts are able to support you in setting up reproducible and automated workflows, from generating 3D tissue models to image-based readout and data analysis.
Automated monitoring of cellular assays
Live-cell imaging lets scientists monitor the dynamic of cellular phenotype particularly when the endpoint of the assay is unknown. Our CELLCYTE X imaging system offers live-cell imaging for online tracking of cell behavior in response to various compounds. The imager contains three fluorescent channels, which enable flexibility with common fluorescent-based measurements used in assay development.
The device is compact and readily fits in an incubator for time-lapse cell imaging. The system significantly increases walk-away time and helps you automate cell-based assays. CELLINK’s cloud-based software delivers remote monitoring without the risk of disturbing an experiment. In addition, our machine-learning algorithms allow for automated analysis of cell proliferation, cell viability, cell migration and other common assays.
Karlsruhe Institute of Technology (KIT)
A premier non contact liquid handling solution designed to provide efficiency and speed to your dispensing processes. The I-DOT can assist in miniaturizing your assays, compound screening or sample prep.
The CELLCYTE X is the premier solution to maximize insights from your cell culture experiments. Designed to fit in most incubators, users can harness the power of live cell imaging and uncover answers to today’s most pressing questions.
Featuring Immediate Drop-on-demand Technology (I-DOT), the lightweight I-DOT Mini optimizes noncontact liquid handling tasks to bring intuitive automation, precision and speed to every lab in a smaller footprint.
Our state-of-the-art systems combine user-friendliness, accessibility and cutting-edge capabilities to enable bioprinting with any cell line, in any geometry and using any biomaterial.
We developed the world’s first universal bioink. Today, hundreds of labs use it in more than 50 countries worldwide. We continue to design innovative bioinks with excellent printability and bioactive properties that guide cellular fate processes.