Anaerobic cell isolation

Automated single-cell isolation from complex microbial samples

Challenges in isolating microbes

A critical step in the process of isolating and studying microbes from complex communities is the efficient separation of single community members to obtain pure cultures. This is complicated by the uneven distribution and high complexity of mixed microbial samples such those from human feces or environmental sources like marine samples and wastewater.

Application Note

Here we introduce a simple workflow for rapid generation of axenic cultures of gut bacteria and generated 1,181 clonal cultures of Escherichia coli and Bacteroides vulgatus and 1,666 clonal cultures of mouse gut bacteria. In the ultimate cultures, MALDI-TOF-MS identified up to 500 isolates, revealing the presence of potentially new species.

b.sight – High-throughput Cultivation Workflow for Isolation of Anaerobic Gut Bacteria

Here we introduce a simple workflow for rapid generation of axenic cultures of gut bacteria and generated 1,181 clonal cultures of Escherichia coli and Bacteroides vulgatus and 1,666 clonal cultures of mouse gut bacteria. In the ultimate cultures, MALDI-TOF-MS identified up to 500 isolates, revealing the presence of potentially new species.
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Anaerobic single-cell isolation made easy

The b.sight™ can readily isolate and deposit a single cell per well in liquid culture media or directly onto solid agar. Due to its small footprint and simple handling, the device can be easily accommodated in anaerobic workstations commonly used for handling strict anaerobes in appropriate conditions.

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b.sight™

Isolate prokaryotic cells in 96- or 384-well plates. Bright field nozzle imaging with 20x magnification. Sort single bacteria for culture, genomics or mass spec.

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