Application Notes

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Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations. |

Printing Alginate Beads: A Technical Note

| Alginate is a commonly used natural biomaterial in tissue engineering and is used extensively as a hydrogel for cell encapsulation and bioactive-delivery systems. The BIO X 3D bioprinter enables rapid and automated alginate bead encapsulations using either the Syringe Pump Printhead or the Electromagnetic Droplet Printhead for customized milli- to micro-meter diameter spherical encapsulations.
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In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins. | |

In Vitro 3D Lung Cancer Model Presents a More Relevant Expression of Junctional Proteins than 2D Cultures (English)

| | In this study, human lung cancer cells were cultured on 2D polystyrene plates and in 3D bioprinted gelatin methacrylate (GelMA) and Matrigel for 14 days to observe spheroid formation, cell morphology and junctional proteins.
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A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days. |

Advanced In Vitro 3D Models to Investigate iPSC Pluripotency and Capillary Network Formation of HUVECs (English)

| A co-culture of human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs) and a monoculture of induced pluripotent stem cells (iPSCs) were separately embedded in a selection of biomaterials for 7 days.
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In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis. |

3D Bioprinting Skin Tissue Models Using Primary Cells

| In this study, a full thickness skin tissue model was bioprinted using the BIO X. The dermis was bioprinted using primary dermal fibroblasts embedded in GelXA SKIN bioink, and the epidermis, containing a high concentration of keratinocytes embedded in ColMA, was deposited on top of the dermis.
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Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X. |

Evaluating Liver Toxicity in Bioprinted Mini Livers

| Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X.
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Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models. |

Comparing Drug Response in 2D Cultures and 3D Bioprinted Tumoroids

| Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models.
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3D bioprinting a syngeneic tumor model of murine lung cancer on the BIO X to evaluate an immune checkpoint inhibitor (PD-1) in a 3D T cell cytotoxicity assay. |

3D Bioprinted Tumor Model for Immuno-oncology Applications

| 3D bioprinting a syngeneic tumor model of murine lung cancer on the BIO X to evaluate an immune checkpoint inhibitor (PD-1) in a 3D T cell cytotoxicity assay.
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