f.sight™
Single-cell dispensing made easy with fluorescent sorting
f.sight™
Single-cell dispensing made easy with flourescent sorting
Tailored for
Benefits
High efficiency
Detetction of low intensity dye or low copy numbers
Laser for red fluorescence available
(mCherry, Texas Red, RFP, Deep Red and more)
Specials
High-resolution objectives
Freely adjustable laser
Integrated plate de-ionizer eliminates electro static charge
Compatible with various PCR plates
Reliable and highly efficient single-cell dispensing
The f.sight™ dispenses both unlabeled and fluorescent cells with the highest efficiency. The novel dual camera system allows you to capture bright field and fluorescence information simultaneously at full resolution.
No electrostatic charge
Deposited within millimeter
Visualization made easy
Both cameras can be controlled independently and the built-in powerful blue laser is freely adjustable. Together, their high dynamic range allows you to visualize even the weakest fluorophores.
Enabling greater insights
You want to use fluorescent dyes to label your products right in or on the cells producing them? Not a problem, the f.sight™ can visualize them, even when there is only few of them.
You also want to process non-fluorescent cells? That’s ok, just switch off the laser and use the f.sight’s bright field sorting mode. Just like with its small brother – the c.sight – cells can then be sorted by morphological criteria.
No risk of bleaching or phototoxicity
Use your standard dyes like GFP, FITC, Calcein AM, CellTracker Green, and others. Thanks to the non-transparent flap, your dyes and cells are protected from ambient light during the process.
Afraid of bleaching or phototoxic effects?
You don’t have to. The laser spot is only illuminating the nozzle. Laser intensity can be freely adjusted. If you run sensitive dyes, reduce the laser intensity and increase the cameras exposure time instead to keep your sample safe.
You work with red fluorescence?
No problem, we can equip your f.sight with a laser for red fluorescence detection so that you can work with dyes like mCherry, Texas Red, RFP, Deep Red and more.
Reliable and highly efficient single-cell dispensing
Our all new software is fast and intuitive. Design your individual fluorescence experiments within minutes. Full-resolution bright field and fluorescence images separately plus an overlay image are all recorded and saved for future analysis and for proof of clonality.
Featured workflow
Our extensive product portfolio offers a range of innovative technologies and instruments for significantly increasing the speed, quality and efficiency of your cell line development workflows.
1. Transfection
2. Cell counting
3. Single-cell cloning & proof of clonality
4. Early suspension culture & upscaling
5. Bioreactor 0.2-20 l
6. Selection of high producer clones
Transfection
Cell counting
Single-cell cloning & proof of clonality
Early suspension culture & upscaling
Bioreactor 0.2-20 l
Selection of high producer clones
Related application notes
We demonstrate an efficient, high-throughput workflow that incorporates the f.sight™ for both selection and cloning of CRISPR/Cas9-edited mesenchymal stem cells (MSCs). Here, MSCs underwent gene editing to knockout the RANKL protein to further enhance the therapeutic capacity of MSCs in regenerative medicine. Cell Line Development | f.sight™
Streamlined Workflow for the Generation of CRISPR-edited Mesenchymal Stem Cell Clones for Regenerative Medicine Applications
Cell Line Development | f.sight™
We demonstrate an efficient, high-throughput workflow that incorporates the f.sight™ for both selection and cloning of CRISPR/Cas9-edited mesenchymal stem cells (MSCs). Here, MSCs underwent gene editing to knockout the RANKL protein to further enhance the therapeutic capacity of MSCs in regenerative medicine.
Download
Download Application Note
f.sight – Fluorescence intensity-based isolation of single cells with assured clonality for CLD workflows
Download
Download Application Note
We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates,
then libraries were prepared with the I-DOT. Single-cell omics | I-DOT | f.sight™
Miniaturization and Automation of a full-length Single-Cell RNA-seq Workflow
Single-cell omics | I-DOT | f.sight™
We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates,
then libraries were prepared with the I-DOT.
Download
Download Application Note
Streamlined Workflow for the Generation of CRISPR-edited Mesenchymal Stem Cell Clones for Regenerative Medicine Applications
f.sight – Fluorescence intensity-based isolation of single cells with assured clonality for CLD workflows
DownloadMiniaturization and Automation of a full-length Single-Cell RNA-seq Workflow
Control the device from outside the cabinet
Simply connect the monitor, keyboard and mouse or even use a remote control terminal
Questions?
We are happy to answer all you questions. Contact us at [email protected].