Next generation microbioreactor for cell line development.
Next generation microbioreactor for cell line development
Bringing production bioreactor conditions to 96-well plates
C.BIRD is a high throughput microbioreactor for parallel cultivations in 96-well plates, bringing production bioreactors environments into 150-1600 µL microbioreactor scale.
Cutting weeks off the cell line development process
The C.BIRD™ enables early identification of the highest producing and most stable clones in order to reduce later passaging steps and efforts of current scaling-up process.
Better predictability of best cell clones
The C.BIRD™ screens and monitors valuable cell culture parameters for hundreds of cell lines from the earliest stages, helping scientists define the best clones sooner.
Application: Cell line development
In the biopharmaceutical industry, many biological drugs are produced in mammalian cells. Cell line development (CLD) is used to determine which cell lines have the highest recombinant protein production and are also stable during large-scale manufacturing.
In the CLD workflow, cell lines undergo incremental scaling of culture from static to shaker flask expansion and further scaling up in bioreactors. Cell culture in early CLD is currently limited to static format as the size of the well plates and number of cells limit the ability to agitate the culture.
Optimize cell culture environments in 96-/24-well plates
- lncrease oxygen transfer rate (OTR) for both adherent cells and suspension cells
- Bring cells into suspension culture (only for suspension cells)
- Continuous mixing for homogenous media composition
- Low shear rate mixing to reduce stress on cell lines
CHO-K1 mAb expressing cell line was used for the study. The cell line was adapted to the culture suspension in a chemically defined and animal-component-free medium (CD Hybridoma medium # 11279-023 by GibCo).
Standard 96-well plates (Eppendorf, Germany) were used for all experiments. Comparison studies were performed with cells cultivated in standard static culture and C.BIRD™ suspension culture in a 37°C, 5% CO2 incubator environment. Resulting cell growth and protein production were compared.
The C.BIRD suspension culture in 96-well plates improves cell proliferation, recombinant protein yield and volume-specific productivity (Qp) compared to traditional static culture. We investigated different initial cell concentrations and measurements were done using multiple instruments to ensure accuracy. In all cases, the C.BIRD with suspension culture exhibited better performance than static culture.
In case 1, the C.BIRD suspension culture achieved a cell density of 4.2×106 cells/ml, but the static culture only reached 1.8×106 cells/ml alter 3 days of culture. In case 2, C.BIRD suspension culture achieved a cell density of 3.1×106 cells/ml, but the static culture only reached 1.9×106 cells/ml alter 5 days of culture.
The C.BIRD™ also demonstrated -2.5-3.8 times higher recombinant protein yield than static culture. (Fig. 3). For the relative volume-specific productivity (Qp), C.BIRD™ suspension culture generated -1.8-2.6 times higher Qp than static culture.
For the relative volume-specific productivity (Qp), the C.BIRD™ suspension culture generated -1.8-2.6 times higher Qp than static culture.
How the C.BIRD works
Our extensive product portfolio offers a range of innovative technologies and instruments for significantly increasing the speed, quality and efficiency of your cell line development workflows.