GelXA LAMININKs

Generate complex tissue models.

With a diverse selection of GelXA LAMININKs, researchers are able to bioprint all cell types (animal, human, progenitor and stem cells) and generate complex tissue models. Laminin proteins are major components of the basal lamina that lines the external surface of cell membranes and play an important role in cell differentiation, migration, adhesion, improving the maintenance and survival of living tissues.

The GelXA LAMININKs include GelXA LAMININK 111, 121, 411, 521 and +. These bioinks are based on the GelXA Bioink and incorporate different laminin isoforms to mimic the basal lamina of natural tissue environments. GelXA LAMININKs maximize cell viability and are a great starting point for the culture of many cell types. Compared to other heterogenous matrices, our laminins’ xenographic source enables better control over the matrix environment.

GELXA Laminink series

LAMININK 521

Supports different tissues types, including pluripotent and embryonic stem cells and induces their self-renewal. Supports many cell types, including cells from pancreas, vascular, nervous and muscular systems.

LAMININK 121

Used as a starting point for culturing many cell types. Expressed during embryonic and early development stages of organ and tissue lining. This laminin variation is mostly found in the placenta, skeletal muscle and kidney tissue.

LAMININK 411

Supports different tissues, including cells from the pancreatic, vascular, immune, nervous and hematopoietic systems. Well-suited for culturing specialized cells, including pancreatic cells and neurons.

LAMININK +

Essential to cell differentiation, migration and adhesion are now available in a stable and easy-to-use bioink. Prints at room temperature and crosslinks with our ionic crosslinking agent or by photocuring.

LAMININK 111

Critical during early development stages of organ and tissue lining. Enhances specialized applications including culturing brain, kidney, liver and intestinal cells. In disease modeling, this bioink can help study Parkinson’s disease and cancer models.

Liver cells and GelXA LAMININK 111

A drug response experiment was conducted on liver tissue models using GelXA LAMININK 111 for the co-culture of HEPG2 and LX2 cells. The bioprinted liver tissue constructs were dosed at Day 0 with different concentrations of a toxic reagent to determine cytotoxicity of the cells over a period of 7 days. Even though the cells demonstrated high cytotoxicity levels after 2 days, they were able to recover from the introduction of the reagent after 5 days.

GELXA LAMININKs in action

Direct functional analysis of the liver tissue model was conducted with histological analysis through immunohistochemical imaging. The collagen type I antibody was used to evaluate the production of collagen type I by the stellate cells in bioprinted co-culture constructs (left image), while the ABCC2 antibody helped to evaluate the transporter multidrug resistance protein 2 (MRP2) in hepatocytes (right image).

scale bars = 100 µm

Collagen type I production in co-cultured HepG2 and LX2 in GelXA LAMININK 111 at Day 7 of culture. Collagen type I expression appears in green, and nuclei stained with DAPI appear in blue. Secondary antibody: Alexa Fluor 488 conjugated.

The ABCC2 (MRP2) antibody staining of hepatocyte HepG2 in co-cultured constructs bioprinted with GelXA LAMININK 111. ABCC2 expression appears in green, and nuclei stained with DAPI appear in blue.

You can find GelXA LAMININK 111 included in our Liver Tissue Model Kits, which make it possible to bioprint liver tissue models and comprehensively analyze cells within the constructs using selective key markers of liver functionality.