3D Cell Culture

Moving toward more physiologically relevant cell cultures

Introduction

Recent advances in the automation of 3D cell culture and fabrication have expanded the number of application areas in which 3D cell culture can used . Whether its drug screening, tissue engineering, disease modeling or cell therapies, 3D cell culture can accelerate discovery efforts. Additionally, 3D cell culture can be used to create tissue models, such as spheroids, micro tissues, organoids and bioprinted constructs, using scaffold-free or scaffold-based methods.

2D VS. 3D CELL CULTURE

Three-dimensional cell cultures offer multiple advantages over 2D cell cultures, including the precise geometrical arrangement of multicellular constructs that can better recapitulate the native 3D human physiology. Previous studies have shown that 3D cultures support more relevant cell-cell interactions and demonstrate differences in cell proliferation, morphology, oxygenation, drug and nutrient uptake, excretion and junction proteins when compared to 2D cultures.

EXTRUSION-BASED BIOPRINTING

Extrusion-based bioprinters, such as the BIO X and BIO X6, offer tremendous flexibility when designing 3D cell culture systems or disease models, and when engineering tissues. See disease models and tissue engineering to learn more. Researchers can choose between a variety of tissue-specific bioinks and fabricate constructs with up to six materials or cell types; they can also precisely control geometries and the spatial patterning of cells or materials in an automated manner. Additionally, extrusion-based bioprinters enable high-throughput fabrication of micro tissues and spheroids within bioinks and co-culture organoids.

LIGHT-BASED BIOPRINTING

Light-based bioprinters, like the Lumen X and the Holograph X, provide superior resolution, allowing users to bioprint vascular networks within bioprinted tissues, to effectively fabricate vascularized organized arrays and to develop co-cultures.

High-Throughput 3D Cell Culture Systems

Creating micro tissues with even greater speed

With even greater speed, bio-dispensing systems such as the I.DOT and I.DOT Mini create scaffold-free and scaffold-based organoids or micro tissues. Common applications include the dispensing of adherent cells into culture well plates or cancer cells for tumor spheroid formation.

NON-CONTACT LIQUID HANDLING TECHNOLOGY

The Immediate Drop on Demand Technology (I.DOT) uses non-contact methods to dispense cells without impacting viability and minimizes dead volume to reduce waste; the I.DOT also offers temperature-controlled fabrication with temperature-sensitive bioinks.

LOW VOLUME DISPENSING IN THE NANOLITERS

Additionally, the I.DOT can be used to dispense other ingredients, such as drugs, growth factors and bioactive compounds with precise nanoliter volumes, which allows for enhanced disease modeling or drug screening with your 3D cell cultures.

AUTOMATE COMPLETE WORKFLOWS

Using live cell-imaging microscopes such as the CELLCYTE X automates complete workflows from fabrication and stimulation to the analysis of your 3D cell cultures. Learn more about live-cell imaging here.

Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X.

Evaluating Liver Toxicity in Bioprinted Mini Livers

Bioprinting mini liver models for hepatotoxicity studies using a novel method of “droplet in droplet” encapsulation on the BIO X.
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Assessing the effectiveness of the I-DOT, a low-volume noncontact liquid dispensing device, to form spheroids using the liquid overlay technique.

Tumor Spheroid Formation Using the I-DOT

Assessing the effectiveness of the I-DOT, a low-volume noncontact liquid dispensing device, to form spheroids using the liquid overlay technique.
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Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models.

Comparing Drug Response in 2D Cultures and 3D Bioprinted Tumoroids

Using the BIO X to evaluate drug efficacy in aggressive forms of breast and pancreatic cancer in 2D cell cultures vs. 3D bioprinted tumor models.
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Related products

BIO X™

The most user-friendly bioprinter in the world thanks to its open system, intelligent and interchangeable printheads. Assists in developing complex tissue constructs, testing new drug compounds and streamline workflows in a range of application areas.

LUMEN X™

Enhancing applications such as microfluidics, cell-laden hydrogels and macroporous structures. Designed to print vasculature with biocompatible blue light, it offers advantages in achieving complex branching and tapering of vessels.

Holograph X™

Laser-based bioprinter able to recreate human physiology at sub-micrometer resolutions within minutes. Fully automated, it accepts any user-designed structure and laser prints transplantable 3D tissue scaffolds for research and therapeutic development.

I.DOT™

A premier non contact liquid handling solution designed to provide efficiency and speed to your dispensing processes. The I.DOT can assist in miniaturizing your assays, compound screening or sample prep.

CELLCYTE X™

The  CELLCYTE X is the premier solution to maximize insights from your cell culture experiments. Designed to fit in most incubators, users can harness the power of live cell imaging and uncover answers to today’s most pressing questions.

BIO X6™

The most flexible six-printhead bioprinting platform on the market and the preferred system for those ready to make history. With the BIO X6 in your lab, you can combine more materials, cells and tools — and get your results sooner — than ever before.