Using Cell Collect Products to Harvest Cells from 3D Constructs
Cell harvesting from 3D bioprinted constructs to perform next-generation sequencing is being optimized with matrix dissociation reagents Cell Collect A and Cell Collect G and the BIO X.
Biomaterials like alginate or gelatin have long been attractive for 3D bioprinting on extrusion-based bioprinters like the BIO X. Researchers turn to these biomaterials for 3D cell culturing and bioprinting because of their high biocompatibility, ease of gelation, shear reversibility and low costs. But for downstream applications, like RT-qPCR, immunoblotting and next-generation sequencing, it is necessary to recover cultured cells from 3D constructs. However, cell culture harvesting from bioinks either involves harsh enzymatic digestion of the surrounding matrix or mechanical dissociation that results in low cell viability and yield. As an alternative, we have formulated two proprietary enzyme-based reagents, Cell Collect A and Cell Collect G, to recover cultured cells from alginate- and gelatin-based bioinks, respectively. In this technical note, we present an optimized cell culture harvesting protocol for using matrix dissociation Cell Collect products to help recover cultured cells from bioprinted constructs. Download our technical note for a step-by-step guide to harvesting cells without compromising cell viability or yield.
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