Application notes

Written by scientists for scientists, these papers cover a wide range of applications and highlight novel ways to optimize one’s research with our devices, technologies and consumables.

We demonstrate an efficient, high-throughput workflow that incorporates the f.sight™ for both selection and cloning of CRISPR/Cas9-edited mesenchymal stem cells (MSCs). Here, MSCs underwent gene editing to knockout the RANKL protein to further enhance the therapeutic capacity of MSCs in regenerative medicine.

Streamlined Workflow for the Generation of CRISPR-edited Mesenchymal Stem Cell Clones for Regenerative Medicine Applications

We demonstrate an efficient, high-throughput workflow that incorporates the f.sight™ for both selection and cloning of CRISPR/Cas9-edited mesenchymal stem cells (MSCs). Here, MSCs underwent gene editing to knockout the RANKL protein to further enhance the therapeutic capacity of MSCs in regenerative medicine.
Download
Download Application Note
We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.

Miniaturization and Automation of a full-length Single-Cell RNA-seq Workflow

We introduce a miniaturized, highthroughput workflow in which single HEK293FT cells were sorted by the f.sight™ into 384-well PCR plates, then libraries were prepared with the I-DOT.
Download
Download Application Note