Application notes

Written by scientists for scientists, these papers cover a wide range of applications and highlight novel ways to optimize one’s research with our devices, technologies and consumables.

The fact that the majority of microbial organisms remain unexplored holds enormous potential to discover new metabolic pathways that can be applied in biotechnological processes or used to develop new therapeutics. We present a workflow for exploring such microbial dark matter, based on single-cell isolation and genetic characterization.

Sequencing Single-cell Genomes of Uncultivated Bacteria in the Human Oral Microbiome

The fact that the majority of microbial organisms remain unexplored holds enormous potential to discover new metabolic pathways that can be applied in biotechnological processes or used to develop new therapeutics. We present a workflow for exploring such microbial dark matter, based on single-cell isolation and genetic characterization.
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Here we introduce a simple workflow for rapid generation of axenic cultures of gut bacteria and generated 1,181 clonal cultures of Escherichia coli and Bacteroides vulgatus and 1,666 clonal cultures of mouse gut bacteria. In the ultimate cultures, MALDI-TOF-MS identified up to 500 isolates, revealing the presence of potentially new species.

B.SIGHT – High-throughput Cultivation Workflow for Isolation of Anaerobic Gut Bacteria

Here we introduce a simple workflow for rapid generation of axenic cultures of gut bacteria and generated 1,181 clonal cultures of Escherichia coli and Bacteroides vulgatus and 1,666 clonal cultures of mouse gut bacteria. In the ultimate cultures, MALDI-TOF-MS identified up to 500 isolates, revealing the presence of potentially new species.
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